WebTailed Duplex DNA Is the Preferred Substrate for Rad51 Protein-Mediated Homologous Pairing Research by A.V. Mazin, E. Zaitseva, P. Sung, and S.C. Kowalczykowski, EMBO J. … Web13 Jun 2005 · We have found that the Tte-UvrD helicase unwinds blunt-ended DNA duplexes as well as substrates possessing 3′ or 5′ ssDNA tails. Tte-UvrD was used to develop a …
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Web9 May 2024 · However, a several hundred-base single-stranded (ss) DNA fragment and a 5′-tailed duplex (TD) accomplished the desired sequence changes without DNA cleavage, and might serve as better donors for the cleaved target DNA than ODNs and plasmid DNAs. Web16 Nov 2024 · Background Single-stranded (ss) DNAs are utilized in various molecular biological and biotechnological applications including the construction of double-stranded … hartley ia nursing home
Tailed duplex DNA is the preferred substrate for Rad51 …
Web1 Mar 2000 · Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing Alexander V. Mazin Division of Biological Sciences, Sections of … Web26 Oct 2024 · Duplex sequencing involves tagging both strands of DNA with random, but complementary, double-stranded primers. The molecules are then amplified, sequenced, and grouped by their tags. Errors that are introduced from PCR or sequencing can be filtered out by looking for discrepancies among molecules with the same tag. WebResearch has shown that TteUvrD is able to displace blunt-ended and 5‘-ssDNA tailed duplex. However, researchers determined, that event if TteUvrD prefers 3‘ssDNA tailed duplexes, its unwinding activity is much lower than seen in E. coli UvrD helicase [4] . hartley ia real estate