Splet03. apr. 2024 · Gene expression and binary interaction techniques are vital tools that shape our understanding of protein complexes. An inherent flaw, however, with most current … Splet20. jun. 2024 · Nicotiana benthamiana leaves transformed with pBiFC-2in1-NN co-expressing either EgAdoMetDC with wildtype PaMiSSP10b (a), or PaMiSSP10b with a …
A 2in1 cloning system enables ratiometric bimolecular …
SpletPubMed Splet01. nov. 2012 · The pBiFC-2in1 vectors were transformed into N. benthamiana mesophyll protoplasts [37] and interaction was analyzed by confocal microscopy 16 h after … grocery stores bear delaware
Binary 2in1 Vectors Improve in Planta (Co)localization …
Splet02. dec. 2013 · Abnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring soluble tau … Splet07. jan. 2024 · Resulting plasmids were sequenced and correct clones used in LR Clonase TM reactions to create pGWB5, pDEST22, or pBiFC-2in1-CC expression vectors. 4.3. … Splet13. maj 2015 · The 2in1 system makes use of the λ-phage recombination sites that are commercially exploited in the MultiSite Gateway system of Life Technologies ... Fig. S3A) … grocery stores ballard wa