Irf7 phosphorylation
WebApr 14, 2011 · IRF7 is a lymphoid-specific factor, which is constitutively expressed in the cytoplasm in B cells, pDCs and monocytes in the spleen, thymus, and peripheral blood lymphocytes, and is potently... WebA, kinetics of IRF7 phosphorylation. 293T cells transfected with 10 μg of an IRF7 expression construct were infected with NDV 12 h later, and nuclear extracts harvested at the …
Irf7 phosphorylation
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WebJun 21, 2013 · Interferon regulatory factor (IRF) 7 is a key regulator of Type 1 IFN production [ 32] and belongs to a family of 9 human IRF genes [ 33 ]. Interestingly, the IRF family underwent co-evolution with NF-κB [ 34 ]. WebJul 25, 2024 · Here, we show that IRF3/IRF7 is transcriptionally active in a phase-separated state and reveal a mechanism for the role of IRF3/IRF7 LLPS-mediated ISRE binding, in which DBD deacetylation is...
WebIRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional … WebAug 11, 2009 · IRF3 and IRF7 activation is mediated by a wide range of phosphorylation events, many of which are directly induced by the IKK-related kinases to coordinate protein dimerization, nuclear translocation, DNA binding, and the induction of IFNβ [8,20-24].
WebApr 2, 2024 · SAMHD1 also interacts with the inhibitor-κB kinase ε (IKKε) and IFN regulatory factor 7 (IRF7), leading to the suppression of the IFN-I induction pathway by reducing IKKε-mediated IRF7 phosphorylation. Interactions of endogenous SAMHD1 with NF-κB and IFN-I pathway proteins were validated in human monocytic cells and primary macrophages. WebJul 29, 2016 · Phosphorylation is a dynamic process in the cell that is in general balanced by kinases and phosphatases. While IKKε was the first kinase identified for IRF7 phosphorylation in 2003 7, the phosphatases that negatively regulate IRF7 phosphorylation and activity have not been identified thus far.
WebSep 1, 2024 · Irf3-/-Irf7-/-MEFs were a gift from Prof. Ping-hui Feng (University of Southern California, U.S.A.). Sendai virus (SeV) and GFP-containing vesicular stomatitis virus (VSV-GFP) were provided by Prof. Hongbin Shu (Wuhan University, China). ... Phosphorylation of Ser82 abolishes the promoter binding ability of IRF3. A&B. Immunoblot analysis of ...
Websignaling pathways downstream of PRRs lead tothe phosphorylation of IFN regulatory factor 7 (IRF7) and IRF3, thus promoting their di-merization and translocation to the nucleus where they activate the transcription of type I IFN genes. IRF3 and IRF7 share some structural and functional similarities, dauntless max weapon levelWebIRF7, once induced, is present in the cytoplasm as an inactive protein that is also phosphorylated on specific C-terminal residues by TBK1 and/or possibly IKKε kinases ( Fitzgerald et al., 2003; Sharma et al., 2003 ). IRF7 has an important role in the regulation of IFNA genes other than A4. dauntless meanWebMar 23, 2001 · IRF7 phosphorylation was blocked by staurosporine (Fig. 3 A, lane 4), a broad-spectrum kinase inhibitor previously shown to block phosphorylation of IRF1 in … dauntless mic not workingWebNov 18, 2024 · IRF3 and IRF7 are critical transcription factors in the innate immune response. Their activation is controlled by phosphorylation events, leading to the … dauntless max reforgeWebMar 6, 2024 · IRF7 is a key transcriptional factor involved in regulating IFN-α gene in lymphoid cells . Phosphorylation of IRF7 is necessary for IFN-α signal transduction, followed by nuclear translocation and activation of type I IFN gene transcription [7, 8]. However, when the expression of type I IFN is dysregulated, adverse immunological effects may ... black actress bookWebFollowing phosphorylation, IRF7 translocates to the nucleus and initiates the transcription of type I and type III IFNs. IRF5 positively regulates the induction of pro-inflammatory cytokines and is critical for IFN-β gene induction while only partially affecting IFN-α induction. IRF8 positively regulates the induction of pro-inflammatory ... dauntless meta builds spreadsheetWebSubsequently, IRF3 and IRF7 are activated (67, 68), and both are important for the expression of type I IFN genes . We examined the activation of the TBK1-IRF3/7 pathway in monocytes stimulated with IMQ or ssRNA40 as compared to vehicle-treated cells (Fig. 8, H and I). The activation of TBK1 was increased by ssRNA40 during the first 30 min of ... dauntless meta builds 2021