Incipient cytorrhysis assay
WebAug 4, 2014 · (A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying … WebApr 6, 2007 · Appressorial turgor was estimated using incipient cytorrhysis (cell collapse) assays ( 6 ). Conidia in 20-μl droplets (1 × 10 6 conidia/ml) were induced to form appressoria on plastic microscope coverslips incubated in a humid chamber for 24 h or 48 h.
Incipient cytorrhysis assay
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WebApr 16, 2024 · (d&e) Incipient-cytorrhysis was performed to compare the appressorial turgor genesis in the 24-h appressoria of strains using glycerol or PEG8000 as the external … WebJul 1, 2024 · Appressorium turgor was measured following the incipient cytorrhysis (cell collapse) assays ( Howard et al., 1991 ). The plastic coverslips were prepared to incubate droplets (20 μl) of conidial suspension (1 × 10 5 conidia/mL). The setup was placed in a humid chamber for 24 h.
WebApr 11, 2014 · We applied incipient cytorrhysis to investigate the appressorial turgor pressure of P. pachyrhizi. Mature appressoria were … WebAntibody Internalization. Antibodies specific for cell surface receptors induce receptor-mediated endocytosis when they bind. They are commonly studied as vehicles for …
WebNational Center for Biotechnology Information WebIn an incipient cytorrhysis (cell collapse) assay using a 1–4 molar concentration of glycerol solution, appressoria of the ΔMomip11 mutant showed increased collapse rates in …
WebJul 28, 2015 · The appressoria formed at 24 h and 48 h post incubation were used in the incipient-cytorrhysis assay to measure the appressorial turgor as described (34). The experiments were replicated three times with more than 200 appressoria were counted for each strain. ... The incipient-cytorrhysis method was used to compare the appressorial …
Web(A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying concentrations (1-4 M). diamond\\u0027s b6WebNov 2, 2015 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5-molar concentration of glycerol solution, as previously described (Foster et al ., 2003 ). Visualization of glycogen and lipid during appressorium development Appressorium formation was induced on the coverslips (Fisher Scientific). diamond\\u0027s b5WebDec 27, 2016 · (C) Incipient cytorrhysis assay to measure appressorium turgor generation in Guy11, Δcds1, and Δchk1Δcds1 mutants in the presence or absence of HU exposure … diamond\\u0027s b1Webincipient cytorrhysis assays indicate that turgor pressure begins to be generated in the appressorium from 16 h (Fig. 1) and penetration of the rice cuticle can occur from 24 h … diamond\u0027s b5WebJan 26, 2016 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5 M glycerol solution as previously described (Foster et al. 2003). The onion penetration assay was performed as previously described (Wang et al. 2013). Onion epidermal strips were isolated and 40 μl of conidial suspension was inoculated onto the adaxial surface. diamond\\u0027s b2WebSep 27, 2024 · Incipient cytorrhysis assay Appressorial turgor was estimated using incipient cytorrhysis assays ( Howard et al., 1991 ). Droplets (20 µL of 1 × 10 5 conidia/mL) of M. … diamond\u0027s b6WebSep 6, 2012 · Appressorial turgor pressure was determined by incipient-cytorrhysis assay reported previously ( Howard et al. 1991 ). The mycelia were placed onto artificial cellophane maintained on water agar plates and incubated at 25 °C, and the samples were microscopically observed at intervals. diamond\u0027s b3