Dhx9 atpase assay

Author: rfzw

August undefined, 2024

WebMay 8, 2024 · Because DHX9-depleted cells exhibit a termination defect (Figures 4 H and 4I), we examined the potential role of DHX9 in preventing R-loop-associated DNA damage. For these studies, we used the Top1 inhibitor camptothecin (CPT), which is known to promote R-loop accumulation and replication- and transcription-associated DNA damage … WebJun 10, 2011 · For competition assays, unlabeled competitor substrates (D-loops, R-loops, forks and G4 structures) were preincubated with DHX9 for 3 min at 25 °C in helicase …

Tissue expression of DHX9 - Staining in testis - The Human Protein …

WebMay 8, 2024 · A top candidate DHX9 helicase promotes R-loop suppression and transcriptional termination. DHX9 interacts with PARP1, and both proteins prevent R … WebDHX9. On the top, protein expression in current human tissue, based on all annotated cell types, is reported with the units not detected (n), low (l), medium (m) and high (h). … flughafenshuttle barcelona

DNA-sensing–independent role of a nuclear RNA …

WebKeywords: ATPase assay, ATA, aurintricarboxylic acid, DHX9, HTS, RNA binding, RNA helicase. Abstract: The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from genome stability and replication, to transcriptional ... WebMar 29, 2024 · d, Luciferase assays show that DHX9 depletion significantly reduces expression of inverted-repeat-Alu-containing 3′ UTR elements, which can be alleviated … WebImportantly, binding of Nup98 to DHX9 stimulates the ATPase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated … flughafen shuttle

DHX9 contributes to the malignant phenotypes of colorectal

WebRNA-seq analysis showed that the Dhx9 deficiency caused an upregulated DNA damage response pathway and Gadd45, Cdkn1a, Cdc25, Wee1, and Myt1 expression to induce … WebNational Center for Biotechnology Information flughafen shirazWebMar 5, 2024 · DExD/H-box RNA helicases (DDX/DHX) are encoded by a large paralogous gene family; in a subset of these human helicase genes, pathogenic variation causes neurodevelopmental disorder (NDD) traits and cancer. DHX9 encodes a BRCA1-interacting nuclear helicase regulating transcription, R-loops, and homologous recombination and … greene public library greene iowa hours

"WebDHX9 has also been shown to directly interact with CBP to aid in recruitment of RNA polymerase II to CRE-containing genes, 46 with the ATPase activity of DHX9 being important for transcriptional induction of genes regulated by a CRE sequence. 47 These data prompted us to test if Nup98 could stimulate CBP-dependent transcription in a … " - Dhx9 atpase assay

Dhx9 atpase assay

Identification of DHX9 as a cell cycle regulated nucleolar ... - Nature

WebWe show that aurintricarboxylic acid, a promiscuous protein-nucleic acid inhibitor prevents DHX9-mediated hydrolysis demonstrating that the assay is also capable of detecting … WebJun 6, 2024 · The RNA helicase DHX9 associates with IGS-rRNA pRNA is a 250–300 nucleotide transcript that derives from processing of the 2-kb …

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WebOct 22, 2024 · DHX9 shares common functions with CIZ1, including interaction with XIST long-non-coding RNA, epigenetic maintenance and regulation of DNA replication. ... Products of GST-CIZ1 interaction assay ... WebNov 1, 2011 · DHX9 is a member of the DExDc helicase family and is a homolog of Drosophila maleless protein, which is essential to regulate X-linked gene expression and the survival of male larvae ().DHX9 …

WebAug 1, 2012 · The goal of this project is to develop HTS assays that will allow discovery of small molecule inhibitors of DHX9, which in turn, can be used in cell and animal models to examine the consequences of inhibition of this protein on cancer cell survival. ... Pelletier, Jerry (2015) Establishment of a Primary Screening Assay for the DHX9 Helicase ... Web13211 Ensembl ENSG00000135829 ENSMUSG00000042699 UniProt Q08211 O70133 RefSeq (mRNA) NM_001357 NM_030588 NM_007842 RefSeq (protein) NP_001348 NP_031868 NP_001392438 Location (UCSC) Chr 1: 182.84 – 182.89 Mb Chr 1: 153.33 – 153.36 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse ATP-dependent …

WebMar 6, 2024 · ATPase Assays. One of the most robust uses of measuring ATPase inhibition is with an ATPase Activity Assay. The protocol developed by BellBrook Labs has been in use for over ten years, with … WebSep 17, 2013 · Our ChIP assays indicate that DHX9 was stably bound to the H-DNA-forming region of the c-MYC promoter sequence. Thus, it is likely that the ChIP assays captured specific interactions between DHX9 and H-DNA in chromatin-plasmid complexes in human cells. ... FANCJ uses its motor ATPase to destabilize protein-DNA complexes, …

WebNov 13, 2024 · The proliferation rates of DHX9-overexpressed or -silenced CRC cells were determined by MTS assay and trypan blue exclusion assay. We found that there was no …

WebMar 29, 2024 · d, Luciferase assays show that DHX9 depletion significantly reduces expression of inverted-repeat-Alu-containing 3′ UTR elements, which can be alleviated with wild-type (WT) DHX9 overexpression ... flughafenshuttle bonnWebCompare DHX9 Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. greene public library iaWebThe RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expr greene public library iowaWebJul 5, 2016 · Initial helicase assays demonstrated double-stranded DNA unwinding activity in bovine DHX9 and RNA unwinding activity with the human orthologue; however, … flughafenshuttle chaniaWebSep 28, 2024 · DHX9 enhanced NF-κB-mediated transactivation in the nucleus, which required its ATPase-dependent helicase (ATPase/helicase) domain, but not the … greene public library riWebDHX9 is part of cluster 34 Non-specific - Transcription with confidence i Confidence is the fraction of times a gene was assigned to the cluster in repeated clustering, and therefore … flughafen shopWebOct 19, 2015 · The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from … greenepublishing.com