WebI have the option of using a biotin-tag, and before I pay $100 more for it over the 6x HIS-tag, I would like a solid reason to avoid nickel columns. Do you have any insight would … WebCys-Streptavidin is a recombinant streptavidin for site-specific conjugation. Like the native streptavidin, Cys-Streptavidin has four identical subunits and binds specifically to biotin with an extremely high affinity. However, the subunit of the Cys-Streptavidin protein has a single cysteine residue at N-terminus for direct conjugation.
Recombinant Mouse FLT1 Protein, HEK293 Cells, His & AVI Tag, …
Molecular Weight: 244 Da Affinity Resin: Avidin/Streptavidin Applications:protein purification, surface plasmon resonance, protein expression. Strengths:can utilize strong affinity for avidin and streptavidin. Limitations: co-elution with other biotinylated proteins can be encountered in mammalian systems. … See more Molecular Weight:0.2–1.6 kDa. 6x-His tag is 0.8 kDa. Size:2–10 histidine residues Tag location:C- or N- terminals, or internal. Affinity Resin: transition metal ions, usually Ni2+ … See more Webof His-tags using Ni2+-NTA (nitrilotriacetic acid) columns, and secondly, the pH-dependent binding of iminobiotin to strep-tavidin (basic pH, K d 1110 M; acidic pH, K d 10 3 M).13 In this method, varying numbers of biotin or iminobiotin conju-gated His-tags (Bio-His-Tag or Ibio-His-Tag, respectively) were rst introduced on to streptavidin (Fig. 1). ion torrent 530 chip
6X His Tag Antibody Biotin Conjugated (200-306-382) Rockland
WebAntibodies that detect 6x His Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Immunohistochemistry. Our 6x His Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Goat, Sheep … WebBiotin-binding plates: Streptavidin or neutravidin: Binds small biotinylated peptides and other small molecules that are difficult to bind by passive adsorption: Fusion-tag binding plates: Glutathione (GST tag binding) or … WebMouse monoclonal 6X His tag® antibody [AD1.1.10] conjugated to Biotin. Validated in WB, ELISA and tested in Species independent. Immunogen corresponding to synthetic peptide. ionto plasma light